Effect of heparin on hemagglutination by equine arteritis virus.

Equine Viral Arteritis

Equine viral arteritis (EVA) is a contagious viral disease of equids caused by equine arteritis virus (EAV), an RNA virus that is classified in the family Arteriviridae. The virus is found in horse populations in many countries worldwide. EVA cannot be differentiated clinically from a number of other respiratory and systemic equine diseases. While laboratory-confirmed outbreaks of EVA have been...

Geographic Distribution of Equine Arteritis Virus Clinical Outcome of Equine Arteritis Virus Infection How Equine

Hemagglutination by equine infectious anemia virus.

Equine infectious anemia (EIA) virus which was propagated on an equine dermal cell line agglutinated guinea pig erythrocytes. Viral fluids containing about 10(7.5) mean tissue culture infective doses/ml showed hemagglutinating (HA) titers ranging from 16 to 32 units/0.05 ml. Results of cesium chloride equilibrium density gradient centrifugation revealed that the hemagglutinin was inseparable fr...

Equine Arteritis Virus Does Not Induce Interferon Production in Equine Endothelial Cells: Identification of Nonstructural Protein 1 as a Main Interferon Antagonist

The objective of this study was to investigate the effect of equine arteritis virus (EAV) on type I interferon (IFN) production. Equine endothelial cells (EECs) were infected with the virulent Bucyrus strain (VBS) of EAV and expression of IFN-β was measured at mRNA and protein levels by quantitative real-time RT-PCR and IFN bioassay using vesicular stomatitis virus expressing the green fluoresc...

Rt-pcr as Effective Detection Method of Equine Arteritis Virus in the Semen of Naturally Infected Stallions

The aim of the study was to present the usefulness of one tube reverse transcriptase polymerase chain reaction assay (RT-PCR) in comparison to virus isolation test (VI) in the detection of equine arteritis virus. The group of 82 semen samples taken from different stallions was examined. Equine arteritis virus was isolated in RK-13 cell monolayers from the seminal plasma of 11 animals. The same ...